Antineuroinflammatory and Neuroprotective Effects of Gyejibokryeong-Hwan in Lipopolysaccharide-Stimulated BV2 Microglia

Figure 2

Effect of Gyejibokryeong-hwan on NO production and iNOS and COX-2 expression in BV2 cells. BV2 cells were pretreated with GBH for 1 h and then stimulated with LPS (100 ng/mL) for 24 h. (a) The levels of NO in the cell culture supernatant were measured by NO detection kit. (b) The level of iNOS was determined by western blot analyses. β-actin was used as a loading control. The data represent three independent experiments. BV2 cells were pretreated with GBH for 1 h and then stimulated with LPS (100 ng/mL) for 6 h. Levels of (c) iNOS and (d) COX-2 mRNA were determined by real-time PCR. GAPDH was used as a loading control. The data represent the mean ± SD of triplicate determinations (one-way ANOVA; p < 0.001 vs. control; p < 0.05, p < 0.01, p < 0.001 vs. LPS-treated control).

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