Antineuroinflammatory and Neuroprotective Effects of Gyejibokryeong-Hwan in Lipopolysaccharide-Stimulated BV2 Microglia

Figure 3

Effect of Gyejibokryeong-hwan on mRNA expression of proinflammatory cytokines in BV2 cells. BV2 cells were pretreated with GBH for 1 h and then stimulated with LPS (100 ng/mL) for 6 h. mRNA expression levels of (a) IL-6, (b) IL-1β, and (c) TNF-α were determined by real-time PCR. GAPDH was used as a loading control. BV2 cells were pretreated with GBH for 1 h and then stimulated with LPS (100 ng/mL) for 16 h. Levels of (d) IL-6, (e) IL-1β, and (f) TNF-α in the cell culture supernatant were measured via ELISA. The data represent the mean ± SD of triplicate determinations (one-way ANOVA; p < 0.001 vs. control; p < 0.05, p < 0.01, p < 0.001 vs. LPS-treated control).