Antineuroinflammatory and Neuroprotective Effects of Gyejibokryeong-Hwan in Lipopolysaccharide-Stimulated BV2 Microglia

Figure 4

Effect of Gyejibokryeong-hwan on mRNA expression of proinflammatory chemokines in BV2 cells. BV2 cells were pretreated with GBH for 1 h and then stimulated with LPS (100 ng/mL) for 6 h. Levels of (a) MIP-1α, (b) MCP-1, (c) IP-10, and (d) RANTES mRNA were determined by real-time PCR. GAPDH was used as a loading control. The data represent the mean ± SD of triplicate determinations (one-way ANOVA; p < 0.001 vs. control; p < 0.05, p < 0.01, p < 0.001 vs. LPS-treated control).

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