Antineuroinflammatory and Neuroprotective Effects of Gyejibokryeong-Hwan in Lipopolysaccharide-Stimulated BV2 Microglia

Figure 5

Effect of Gyejibokryeong-hwan on the phosphorylation of MAPK and Akt and NF-κB activity in BV2 cells. (a) BV2 cells were pretreated with GBH for 1 h and then stimulated with LPS (100 ng/mL) for 15 min. Levels of p-JNK, JNK, p-Erk, Erk, p-p38, p38, p-Akt, and Akt were determined by western blot analyses. β-actin was used as a loading control. (b) BV2 cells were pretreated with GBH for 1 h and then stimulated with LPS (100 ng/mL) for 1 h. Nuclear extracts were analyzed by western blot analysis using an NF-κB p65 antibody. PCNA was used as a loading control. The data represent three independent experiments. The data are expressed as the mean ± SD of triplicate determinations (one-way ANOVA; p < 0.001 vs. control; p < 0.01, p < 0.001 vs. LPS-treated control).

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