Antineuroinflammatory and Neuroprotective Effects of Gyejibokryeong-Hwan in Lipopolysaccharide-Stimulated BV2 Microglia

Figure 6

Effect of Gyejibokryeong-hwan on IL-10 production and HO-1 expression in BV2 cells. (a) BV2 cells were pretreated with GBH for 1 h and then stimulated with LPS (100 ng/mL) for 16 h. Levels of IL-10 in the cell culture supernatant were measured via ELISA. (b) BV2 cells were pretreated with GBH for 1 h and then stimulated with LPS (100 ng/mL) for 6 h. HO-1 mRNA levels were determined by real-time PCR. GAPDH was used as a loading control. (c) BV2 cells were pretreated with GBH for 1 h and then stimulated with LPS (100 ng/mL) for 12 h. The level of HO-1 was determined by western blot. β-actin was used as a loading control. (d) BV2 cells were pretreated with GBH for 6 h. Nuclear extracts were analyzed by western blot using an NRF2 antibody. PCNA was used as a loading control. BV2 cells were pretreated with GBH for 30 min. Levels of p-CREB and CREB were determined by western blot analyses. β-actin was used as a loading control. The data represent three independent experiments. The data are expressed as the mean ± SD of triplicate determinations (one-way ANOVA; p < 0.05, p < 0.01, p < 0.001 vs. control; p < 0.001 vs. LPS-treated control).

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