Antineuroinflammatory and Neuroprotective Effects of Gyejibokryeong-Hwan in Lipopolysaccharide-Stimulated BV2 Microglia

Figure 7

HO-1 mediates the effect of Gyejibokryeong-hwan on NO production and mRNA expression of proinflammatory cytokines in BV2 cells. (a) BV2 cells were pretreated with GBH for 1 h in the presence or absence of SnPP (10 μM) and then stimulated with LPS (100 ng/mL) for 24 h. Levels of NO in the cell culture supernatant were measured by an NO detection kit. BV2 cells were pretreated with GBH for 1 h in the presence or absence of SnPP (10 μM) and then stimulated with LPS (100 ng/mL) for 6 h. Levels of (b) iNOS, (c) COX-2, (d) IL-6, (e) IL-1β, and (f) TNF-α mRNA were determined by real-time PCR. GAPDH was used as a loading control. The data represent the mean ± SD of triplicate determinations (one-way ANOVA; p < 0.001 vs. control; p < 0.001 vs. LPS-treated control; ^†††p < 0.001 vs. LPS+GBH).

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