Analytical Method Validation of Gamijakyakgamchobuja-Tang (KCHO-1, Mecasin) Preparation

Table 10

Stability of the dosing formulations for 7 days under refrigeration.

(a) Curcumin

Day	Concentration of the dosing formulation (μg/mL)	Measured concentration			Mean (μg/mL)	SD	CV (%)	Accuracy (%)	Variation (%)
		(μg/mL)
		No.1	No.2	No.3
0	39.5	46.52	45.79	46.33	46.21	0.38	0.82	116.99	-
	158.0	186.52	180.49	184.37	183.79	3.06	1.66	116.32	-
7	39.5	37.92	38.65	36.57	37.71	1.06	2.80	95.48	-18.39
	158.0	143.93	152.77	152.53	149.74	5.04	3.37	94.77	-18.53

(b) Glycyrrhizic Acid

Day	Concentration of the dosing formulation (μg/mL)	Measured concentration			Mean (μg/mL)	SD	CV (%)	Accuracy (%)	Variation (%)
		(μg/mL)
		No.1	No.2	No.3
0	305.0	234.75	240.17	244.67	239.86	4.97	2.07	78.64	-
	1220.0	961.40	959.78	971.12	964.10	6.13	0.64	79.02	-
7	305.0	277.24	273.06	264.09	271.46	6.72	2.48	89.00	13.18
	1220.0	1099.67	1165.43	1119.48	1128.19	33.73	2.99	92.47	17.02

(c) Salvianolic Acid B

Day	Concentration of the dosing formulation (μg/mL)	Measured concentration			Mean (μg/mL)	SD	CV (%)	Accuracy (%)	Variation (%)
		(μg/mL)
		No.1	No.2	No.3
0	985.0	932.67	944.18	948.37	941.74	8.13	0.86	95.61	-
	3940.0	3762.21	3675.21	3755.96	3731.13	48.53	1.30	94.70	-
7	985.0	996.37	991.24	955.72	981.11	22.14	2.26	99.61	4.18
	3940.0	3879.51	4111.82	3986.46	3992.60	116.28	2.91	101.34	7.01