Analytical Method Validation of Gamijakyakgamchobuja-Tang (KCHO-1, Mecasin) Preparation

Table 6

Accuracy and precision of intraday variation.

(a) Curcumin

Concentration of dosing formulation (μg/mL)	Measured concentration (μg/mL)			Mean (μg/mL)	SD	CV (%)	Accuracy (%)
	No.1	No.2	No.3
39.5	46.52	45.79	46.33	46.21	0.38	0.82	116.99
158.0	186.52	180.49	184.37	183.79	3.06	1.66	116.32

(b) Glycyrrhizic Acid

Concentration of dosing formulation (μg/mL)	Measured concentration (μg/mL)			Mean (μg/mL)	SD	CV (%)	Accuracy (%)
	No.1	No.2	No.3
305.0	234.75	240.17	244.67	239.86	4.97	2.07	78.64
1220.0	961.40	959.78	971.12	964.10	6.13	0.64	79.02

(c) Salvianolic Acid B

Concentration of dosing formulation (μg/mL)	Measured concentration (μg/mL)			Mean (μg/mL)	SD	CV (%)	Accuracy (%)
	No.1	No.2	No.3
985.0	932.67	944.18	948.37	941.74	8.13	0.86	95.61
3940.0	3762.21	3675.21	3755.96	3731.13	48.53	1.30	94.70