Analytical Method Validation of Gamijakyakgamchobuja-Tang (KCHO-1, Mecasin) Preparation

Table 7

Stability of treated sample in the autosampler.

(a) Curcumin

Title	Concentration of the dosing formulation (μg/mL)	Measured concentration			Mean (μg/mL)	SD	CV (%)	Accuracy (%)	Variation (%)
		(μg/mL)
		No.1	No.2	No.3
Start	39.5	46.52	45.79	46.33	46.21	0.38	0.82	116.99	-
	158.0	186.52	180.49	184.37	183.79	3.06	1.66	116.32	-
End	39.5	47.06	46.57	46.59	46.74	0.28	0.59	118.33	1.15
	158.0	186.74	183.55	185.76	185.35	1.63	0.88	117.31	0.85

(b) Glycyrrhizic Acid

Title	Concentration of the dosing formulation (μg/mL)	Measured concentration			Mean (μg/mL)	SD	CV (%)	Accuracy (%)	Variation (%)
		(μg/mL)
		No.1	No.2	No.3
Start	305.0	234.75	240.17	244.67	239.86	4.97	2.07	78.64	-
	1220.0	961.40	959.78	971.12	964.10	6.13	0.64	79.02	-
End	305.0	241.22	242.24	243.27	242.24	1.03	0.42	79.42	0.99
	1220.0	943.30	956.29	939.06	946.22	8.98	0.95	77.56	-1.85

(c) Salvianolic Acid B

Title	Concentration of the dosing formulation (μg/mL)	Measured concentration			Mean (μg/mL)	SD	CV (%)	Accuracy (%)	Variation (%)
		(μg/mL)
		No.1	No.2	No.3
Start	985.0	932.67	944.18	948.37	941.74	8.13	0.86	95.61	-
	3940.0	3762.21	3675.21	3755.96	3731.13	48.53	1.30	94.70	-
End	985.0	945.25	965.36	951.74	954.12	10.26	1.08	96.86	1.31
	3940.0	3758.11	3726.34	3733.48	3739.31	16.67	0.45	94.91	0.22