The Combination of Mulberry Extracts and Silk Amino Acids Alleviated High Fat Diet-Induced Nonalcoholic Hepatic Steatosis by Improving Hepatic Insulin Signaling and Normalizing Gut Microbiome Dysbiosis in Rats
Figure 2
Liver morphological and histological differences. (a) The liver size and shape. (b) Staining with hematoxylin and eosin (H-E) in the liver paraffin embedded section (magnification: X200). (c) Staining with PAS in the liver paraffin embedded section (magnification: X200). (d) Scores of the nucleus and cell structure, cell arrangement, macrophage infiltration, and glycogen deposition. Each bar or dot and error bar represents the mean ± SD (n=10). Rats consumed high fat diets containing 0.2% (low dosage, -L) and 0.6% (high dosage, -H) of the mixture of mulberry extracts and silk amino acid (MS) and 0.6% cellulose (disease-control) for 12 weeks. MS1:3 and MS1:5 mixture contained 1:3 and 1:5 of mulberry extracts and silk amino acid, respectively. Sham rats were fed the same diet with the disease-control group as the normal-control. Bars with different letters were significantly different among the groups by Tukey test at P<0.05.