DNA Markers to Discriminate Cannabis sativa L. ‘Cheungsam’ with Low Tetrahydrocannabinol (THC) Content from Other South Korea Cultivars Based on the Nucleotide Sequences of Tetrahydrocannabinolic Acid Synthase and Putative 3-Ketoacyl-CoA Synthase Genes

Figure 2

Dendrogram based on the nucleotide sequences of tetrahydrocannabinolic acid (THCA) synthase genes of samples listed in Table 1 and deposited in NCBI GenBank. The deposited nucleotide sequences of THC synthase in drug-type Cannabis (AB212829.1, AB212832.1, AB212834.1, AB212835.1, AB2128837.1, and AB212838.1) and hemp (AB212830.1, AB212831.1, AB212833.1, AB212836.1, AB212839.4, AB212840.1, and AB212841.1) were used to construct the dendrogram. As outer-groups of a dendrogram, nucleotide sequences of genes encoding CBDA synthase of C. sativa (AB292682.1) and berberine bridge enzyme of E. californica (AF005655.1) were used.