Chlorella vulgaris Modulates Genes and Muscle-Specific microRNAs Expression to Promote Myoblast Differentiation in Culture

Table 1

Sequence of primers used in gene expression analysis.

Gene	Forward primer	Reverse primer
GAPDH	5′-TCCCTGAGCTGAACGGGAAG-3′	5′-GGAGGAGTGGGTGTCGCTGT-3′
Pax7	5′-GTGCCCTCAGTGAGTTCGAT-3′	5′-GTTCCGACTCCACATCCGAG-3′
Myf5	5′-TCACCTCCTCAGAGCAACCT-3′	5′-ATTAGGCCCTCCTGGAAGAA-3′
MyoD1	5′-AGGGGCTAGGTTCAGCTTTC-3′	5′-GCTCTGGCAAAGCAACTCTT-3′
MYOG	5′-CAGTGCCATCCAGTACATCG-3′	5′-AGGTTGTGGGCATCTGTAGG-3′
PTEN	5′-ACTTGAAGGCGTATACAGGAC	5′-AATGTCTTTCAGCACAAAGAT
	CA-3′	TGTA-3′
IGF1R	5′-TGGAGTGCTGTATGCCTCTG-3′	5′-CCCTTGGCAACTCCTTCATA-3′
MEF2C	5′-GGGGACTATGGGGAGAAAAA-3′	5′-ACAGCTTGTTGGTGCTGTTG-3′
MYH2	5′-CAAACATGAGAGGCGAGTGA-3′	5′-CTGGAGCTTGCGGAATTTAG-3′
TNNT1	5′-TGGAGCTGCAGACACTCATC-3′	5′-CTTGGCCTCTTCCTCTTCCT-3′