The expressions of TRPV1 and EP4 in bladder. EP4 (red) and TRPV1 (green) innervation in lower urinary bladder. EP4-IR and TRPV1-IR signals were weak in the control group. EP4-IR was primarily localized to the detrusor muscle, and TRPV1-IR primarily passed through the suburothelium and smooth muscle cells following CYP treatment. Yellow dotted lines delineated the boundary of the urothelium. Scale bar 50 Î¼m in the panels.