Comparison of the Effects of Yokukansan and Yokukansankachimpihange on Glutamate Uptake by Cultured Astrocytes and Glutamate-Induced Excitotoxicity in Cultured PC12 Cells

Figure 3

Effect of CUP or PT on TD-induced decrease in Glu uptake by cultured astrocytes. Astrocytes were cultured for 5 days in (1) Ctrl, thiamine-containing normal medium; (2) T(−), TD medium, or (3) T(−) medium containing CUP ((a) 2–60 μg/mL) or PT ((b) 2–60 μg/mL). Glu concentration of cultured Glu-containing medium (100 μM) was evaluated after 5 h. Data are presented as the mean ± SEM (n = 6). ^†††P < 0.001 vs. Ctrl; P < 0.01 and P < 0.001 vs. Glu: one-way ANOVA + Dunnett’s test.

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