Comparison of the Effects of Yokukansan and Yokukansankachimpihange on Glutamate Uptake by Cultured Astrocytes and Glutamate-Induced Excitotoxicity in Cultured PC12 Cells

Figure 5

Effect of YKS or YKSCH on Glu-induced PC12 cell death. PC12 cells were incubated for 24 h in media containing 8 mM Glu + YKS (10–300 μg/mL) or YKSCH (14–420 μg/mL). The control medium (Ctrl) contained no Glu. (a1, a2) Survival rate calculated as %MTT activity relative to control PC12 cells (n = 6). (b1, b2) Amelioration rate as determined from survival rate. (c) 30% effective dose (ED₃₀) as calculated from the regression equation for the amelioration rate. (d) Comparison of the average ED₃₀ between YKS and YKSCH. Data are presented as the mean ± SEM ((a1), (a2), (b1), and (b2): n = 6; (d): n = 5). ^†††P < 0.001 vs. Ctrl; P < 0.01, and P < 0.001 vs. Glu: one-way ANOVA + Dunnett’s test.