Effect of Tripterygium wilfordii Polycoride on the NOXs-ROS-NLRP3 Inflammasome Signaling Pathway in Mice with Ulcerative Colitis

Figure 4

The mRNA expression of NLRP3, ASC, and caspase-1 in the colon tissues. Bars of the three colors, respectively, indicate the mRNA expression of NLRP3, ASC, and caspase-1 in the colon tissues. Mice in the model control, low TWP, middle TWP, and high TWP groups were provided a 5% DSS solution for one week. Meanwhile, the low TWP, middle TWP, and high TWP groups received different doses of TWP for 2 weeks. At the end of the experiment, the mouse colon tissues of each group were taken for the detection of the mRNA expression of NLRP3, ASC, and caspase-1 via real-time PCR. The results showed that the mRNA expression of NLRP3, ASC, and caspase-1 in the colon tissues of the different TWP groups was significantly lower than that of the model control group and higher than that of the normal control group. The mRNA expression of NLRP3, ASC, and caspase-1 decreases with the increased dose of TWP, and the differences among the TWP groups were significant. Each bar represents mean ± SD (n = 9); vs. model; ^† vs. low TWP; ^▶ vs. middle TWP.