Research Article
Downregulation of Aquaporin 3 Mediated the Laxative Effect in the Rat Colon by a Purified Resin Glycoside Fraction from Pharbitis Semen
Figure 3
RFP activated NF-κB pathway and their downstream proteins. (a) The expressions of phosphorylated NF-κB (p-p65) in nuclear fractions and cytosolic COX-2 were subjected to western blot analysis. PCNA and GAPDH were used as loading controls of nuclear fraction and cytosolic fraction respectively. P < 0.05, P < 0.001 vs control group. (b) Immunohistochemical technique was used to analyze the expression of p-p65 and COX-2 in the colon. The p-p65 and COX-2 positive result presents brown, low resolution (20 x 2.5, bars: 100 μm) and amplifying image (20 x 10, bars: 25 μm). (c) The PGE2 content was detected using the ELISA method. The control group was indicated as 100%. The data represented the means ± SDs for six rats. P < 0.05, P < 0.01, P < 0.001 vs control group. (d) The rats colons after RFP treatment were stained with H&E, low resolution (20 x 2.5, bars: 100 μm) and amplifying image (20 x 10, bars: 25 μm).
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