Downregulation of Aquaporin 3 Mediated the Laxative Effect in the Rat Colon by a Purified Resin Glycoside Fraction from Pharbitis Semen

Figure 4

NF-κB and COX-2 inhibitors suppressed RFP-induced laxative effect. (a, b, c, d) Pretreatment of rats by intraperitoneal injection of NF-κB inhibitor BAY 11-7082 (BAY) (10 mg / kg) or COX-2 inhibitor indomethacin (20 mg/kg) for 1 h and further treatment with RFP (125 mg/kg). (a) After 6 h administration with RFP, rats were autopsied after sacrifice. Macroscopic image of the colon was observed. (b) Rat stool samples were collected 6 hours after RFP administration and fecal water content was measured, P < 0.001 or ns vs control group. (c) Protein expressions of AQP3, COX-2 and p-P65 were analyzed by western blot analysis. PCNA and GAPDH were used as an equal loading control. The data represented the means ± SDs for three experiments, P < 0.05, P < 0.001 or ns vs control group. (d) The levels of PGE₂ were measured with ELISA methods and the control group was presented as 100%, P < 0.001 or ns vs control group.

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