Keratinocyte Growth Factor-2 Is Protective in Oleic Acid-Induced Acute Lung Injury in Rats
Figure 5
Immunohistochemical staining to determine Claudin-5, ZO-1, and VE cadherin expression in rat lung tissue. (a) Claudin-5 expression in the lung tissue of the control group was strongly positive (dark brown); ALI group Claudin-5 expression was negative in lung tissue (light yellow); Claudin-5 expression was weakly positive (brown) in lung tissue of ALI+KGF-2 group; ZO-1 expression in lung tissue of control group strong positive (dark brown); ZO-1 expression was negative in the lung tissue of ALI group (light yellow) and showed that ZO-1 expression was weakly positive in lung tissue of ALI+KGF-2 group (brown yellow); VE cadherin expression was strongly positive in the lung tissue of the control group (dark brown); VE cadherin expression was negative in the lung tissue of the ALI group (light yellow); ALI+KGF-2 group lung tissue VE cadherin expression is weakly positive (brownish yellow). Medium magnification. Positive expression of brown stained strip is indicated by the arrow; (b) statistical analysis of positive expression of each group (< 0.01 versus control group, #<0.01 versus ALI group, and Δ< 0.05 versus ALI group ).