Evidence-Based Complementary and Alternative Medicine / 2019 / Article / Fig 4

Research Article

Extract of Rhus verniciflua Stokes Induces p53-Mediated Apoptosis in MCF-7 Breast Cancer Cells

Figure 4

Effect of RVSE on p53 and p21. (a) and (c) Cells (5 × 105/mL) were treated with 200, 300, or 400 μg/mL RVSE for 24 h. (b) p53 levels (time: 0–24 h) in the lysate of cells treated with 400 μg/mL RVSE were determined. Western blot analysis of the (a) and (b) p53 and (c) p21 protein level; β-Actin was used as the loading control. A representative of three blots yielding similar results is shown. Relative levels (protein vs.β-actin) were determined by densitometry. Data are means ± SD of three independent experiments. P < 0.05, significant difference from the control.