Qualitative and Quantitative Analysis for the Chemical Constituents of Tetrastigma hemsleyanum Diels et Gilg Using Ultra-High Performance Liquid Chromatography/Hybrid Quadrupole-Orbitrap Mass Spectrometry and Preliminary Screening for Anti-Influenza Virus Components

Figure 5

Antiviral determination. MDCK cells were infected with recombinant influenza virus PR8-NS1-Gluc at an moi of 0.01 PFU/cell, and the infected cells were treated with ZJ2 and other extract samples of T. hemsleyanums at indicated concentrations during virus propagation. (a) ZJ2 inhibits influenza virus replication in a dose dependent manner. (b) Dose-response curve of ZJ2 against influenza virus replication and on cell viability. (c) 17 extract samples of T. hemsleyanums collected from different districts as well as ZJ2 show similar antiviral activities against influenza virus. Mean and SEM of three independent experiments were shown. p<0.001, Student’s t-test. There are 18 batches of T. hemsleyanums in this figure. Among them, FJ1, FJ2, and FJ3 are three batches from Fujian, GZ1, GZ2, and GZ3 are from Guizhou, ZJ1, ZJ2, and ZJ3 are from Zhejiang, HB1, HB2, and HB3 are from Hubei, YN1, YN2, and YN3 are from Yunnan, and GX1, GX2, and GX3 are from Guangxi. Their information can be found in the Supplementary Materials (available here).

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