Long-Term Potentiation of Prelimbic Cortex Ascribed to Heat-Sensitization Responses of Moxibustion
Figure 3
MCAO model increased the number of pCofilin positive (pCof+) spines, and moxibustion-induced heat-sensitization responses in rat’s model characterized by tail temperature increase had enhanced this effect. PCof+ spines were counted and values for a rat in a given cohort were normalized to the mean score for ischemic control rats in that cohort. (a) MCAO rats either or not treated with suspended moxibustion exercise (TTI, non-TTI, and C groups) had more pCof+ spines than did normal rats (N group); this effect was absent in sham rats (S group); differences in pCof+ puncta between TTI versus non-TTI or C groups were significant. (b) The increase in numbers of pCof+ puncta in MCAO rats either or not treated with suspended moxibustion exercise was not accompanied by an increase in total number of PSD-95 puncta. (c) The number of PSD-95 puncta that were colocalized with pCofilin immunoreactivity was expressed as a percentage of the total PSD-95 puncta for each rat in the study (values were then normalized to the within-cohort ischemic control rat mean). The C group had a higher percentage of PSD-95 puncta colocalized with pCofilin than did either N or S groups; differences in pCof+ puncta colocalized with pCofilin between TTI versus non-TTI or C groups were also significant. Data were expressed as mean ± SEM. # p<0.05 vs. non-TTI and C groups; p<0.001 vs. N and S groups.