Evidence-Based Complementary and Alternative Medicine

Research Article

Methanolic Extract of Artemia salina Eggs and Various Fractions in Different Solvents Contain Potent Compounds That Decrease Cell Viability of Colon and Skin Cancer Cell Lines and Show Antibacterial Activity against Pseudomonas aeruginosa

Table 1

Major compounds identified in the methanolic crude extract and the hexane and chloroform fractions of Artemia salina eggs. N.D: not determined.


Methanolic crude extract

S.No	name of compound	nature of compound	molecular formula	molecular weight (g/mol)	RT (min)	area (%)	% of total	established activity

1	Dibutyl phthalate	phthalate	C₁₆H₂₂O₄	278.34348	28.932	0.96	0.957	anticancer, antibacterial [25–27, 29]

2	9-octadecenoic acid, methyl ester	unsaturated fatty acid methyl ester	C₁₉H₃₆O₂	296.4879	31.592	0.65	0.648	antioxidant, anticancer [28, 30]

3	Oleic acid	fatty acid	C₁₈H₃₄O₂	282.4614	32.275	1.97	1.969	antitumor, antibacterial [31–34]

4	di(2-ethylhexyl)phthalate	phthalate	C₂₄H₃₈O₄	390.55612	41.927	2.15	2.152	anti-leukemic [37]

Hexane fraction

S.No	name of compound	nature of compound	molecular formula	molecular weight (g/mol)	RT (min)	area (%)	% of total	established activity

1	Phenol, 2, 4-bis(1, 1-dimethylethyl)	phenol	C14H22O	206.32388	19.791	0.03	0.034	antifungal, antibacterial [42, 43]

2	2-cyclohexen-1-one, 2, 4, 4-trimethyl-3-(3-oxo-1-butenyl)	ketone	C₁₃H₁₈O₂	206.2808	23.672	0.15	0.057	anti-inflammatory [44]

3	1-mercapto-2-heptadecanone	thiol	C₁₇H₃₄OS	286.51626	35.623	0.80	0.800	N.D

4	Methyl 4-(7,7-dimethyl-2,5-dioxo-1,2,3,4,5,6,7,8-octahydroquinolin-4-yl)benzoate	quinoline	C₁₉H₂₁NO₄	327.38	38.675	0.53	0.527	N.D

5	Methyl (E)-4′-ethyldiene-3-oxo-2a¹,4′,5′,8a′-tetrahydro-2′H,7′H-spiro[indoline-2,8′-methanofuro4,3,2-hi]indolizine]-2a′(3′H)-carboxylate	indole	C₂₁H₂₂N₂O₄	366.42	44.520	0.07	0.070	N.D

Chloroform fraction

S.No	name of compound	nature of compound	molecular formula	molecular weight (g/mol)	RT (min)	area (%)	% of total	established activity

1	Indole-3-carboxaldehyde	indole	C₉H₇NO	145.15798	26.698	0.25	0.258	antimycobacterial and anticancer [38]

2	Gancidin W	pyrazine	C₁₁H₁₈N₂O₂	210.27282	28.661	0.46	0.461	antimalarial [45]

3	Naloxone	thebaine derivative	C₁₉H₂₁NO₄	327.37434	38.534	1.55	1.555	opioid receptor antagonist, anticancer [39, 40]

4	methyl 1,2,3,4,4a,9,10,10a-octahydro-7-isopropyl-1,4a-dimethylphenanthrene-1-carboxylate	phenanthrene carbaldehyde	C₂₀H₂₈O₂	314.22	39.248	1.95	1.947	anticancer [41]

5	Cholest-5-en-3-ol (3.beta.)	cholesterol	C₂₇H₄₆O	386.65354	47.762	7.20	7.201	N.D