Screening Five Qi-Tonifying Herbs on M2 Phenotype Macrophages

Figure 3

Effect of TFRG on expression of M2-induced genes and M1 marker iNOS in IL-4/IL-13-induced murine RAW264.7 cells. Effect of TFRG on FIZZ1 (a), YM1 (b), and CD206 (c) mRNA in IL-4/IL-13-induced murine RAW264.7 cells. RAW264.7 macrophages were incubated with pretreatment of 25 and 50μg/mL TFRG and stimulated with IL-4 (20ng/mL)/IL-13 (20ng/mL) for 24h. Values are presented as the mean ± standard deviation from three replicates. ^##P < 0.01 versus C group; < 0.05 versus M2 group; < 0.01 versus M2 group. (d) Effect of TFRG on iNOS protein in IL-4/IL-13-induced murine RAW264.7 cells. RAW264.7 macrophages were incubated with pretreatment of 25 and 50μg/mL TFRG and stimulated with IL-4 (20ng/mL)/IL-13 (20ng/mL) for 24h. Values are presented as the mean ± standard deviation from three replicates. ^##P < 0.01 versus C group; <0.01 versus M2 group.

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