Screening Five Qi-Tonifying Herbs on M2 Phenotype Macrophages
Table 3
Effect of herb extracts and ingredients on Arg-1 mRNA expression and cell viability.
Candidates
Dose
Viability (%)
Inhibition (%)
Ethanol extract of GC
100
105.81±4.97
48.21
TFRG
100
107.54±4.98
92.34
Glabridin
50
105.02±2.00
84.38
Isoliquiritin apioside
50
104.05±1.74
53.75
Liquiritin apioside
50
106.79±6.20
41.59
Glycyrrhizic acid ammonium salt
50
94.07±4.36
-
Isoliquiritin
50
97.89±5.25
-
Liquiritin
50
113.24±7.94
-
Ethanol extract of RS
100
92.96±5.52
90.81
Total saponins of RS
100
108.02±7.72
84.30
20(S)-Ginsenoside Rg3
50
95.94±4.30
-
Ginsenoside Rg1
50
97.82±2.08
-
Ginsenoside Rf
50
109.62±5.32
15.06
Lysionotin
50
95.42±2.57
50.98
Notoginsenoside
50
94.08±5.36
-
Ethanol extract of DCXC
100
106.66±2.38
87.09
Cordycepin
50
95.83±1.64
84.40
Ethanol extract of HQ
100
98.86±3.29
65.54
Astragaloside IV
50
106.41±1.86
55.14
Calycosin-7-glucoside
50
98.55±0.97
32.15
Calycosin
50
110.52±6.19
77.51
Ethanol extract of CWJ
100
95.37±8.50
80.65
Eleutheroside B
50
98.97±8.25
30.08
Hederasaponin B
50
98.33±7.20
41.51
Eleutheroside E
50
99.08±1.01
20.52
dosage of herb extracts and fractions was at 100 μg/Ml; the dosage of ingredient was at 50 μM. cytotoxicity: MTT assay was performed to determine cell cytotoxicity of unstimulated RAW264.7 cells treated with herb extracts. Untreated group was considered as 100%. inhibition of Arg-1 expression: qRT-PCR was carried out to measure the production of Arg-1 mRNA in IL-4/IL13-induced RAW264.7 cells in the presence of 100 μg/mL herb extracts and fractions or 50 μM ingredients.
