The Cytotoxic Properties of Baeckea frutescens Branches Extracts in Eliminating Breast Cancer Cells
Figure 1
Effects of B. frutescens branches extracts on MCF-7 cell viability. (a) Cytotoxicity was determined using cell viability assay and the IC50 value was calculated as half maximal percentage of cell viability inhibition and it is indicated by the red horizontal line. MCF-7 cells were treated with either (i) WB, (ii) B90, (iii) B70, or (iv) B50 or (v) etoposide, which served as positive control for 72 h. Data is expressed as mean ± standard error mean based on six independent experiments with triplicate wells for each concentration. (b) IC50 values of B90, B70, B50, and WB against MCF-7 and MDA-MB 231 cells after 24, 48, and 72 h of incubation. (c) Normal mammary epithelial cells (MCF-10A) were treated with either B90, B70, or B50 at three different concentrations: lower than IC50 value (low), IC50 value (IC50), and higher than IC50 value (high) for 72 h. Data is expressed as mean ± standard error mean based on three independent experiments with triplicate wells for each concentration.