Formononetin Enhances the Tumoricidal Effect of Everolimus in Breast Cancer MDA-MB-468 Cells by Suppressing the mTOR Pathway

Figure 2

Sensitivities of MDA-MB-468 cells to formononetin and everolimus. An MTT assay was performed to determine the cell number, as described in Section 2. MDA-MB-468 cells were incubated in formononetin at 50, 100, 150, 200, and 250 µmol/L (a) and everolimus at 1, 10, 100, 1000, and 2000 nmol/L (b) for 12, 24, and 48 h. (c) Both everolimus alone or formononetin and everolimus combined inhibited cell growth for 48 h. Values are means ± SE from 3 independent experiments. p < 0.05 versus control. ^#p<0.05 versus both everolimus and formononetin alone.

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(b)

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