Formononetin Enhances the Tumoricidal Effect of Everolimus in Breast Cancer MDA-MB-468 Cells by Suppressing the mTOR Pathway

Figure 3

Effect of everolimus alone or both formononetin and everolimus on apoptosis in MDA-MB-468 cells. MDA-MB-468 cells were treated with formononetin (150 µmol/L), everolimus (100 nmol/L), or both for 48 h. Cells were subsequently stained for annexin V-PI (a) and PI only (b) followed by flow cytometric analysis. (A) and (E) control, (B) and (F) formononetin (150 µmol/L), (C) and (G) everolimus (100 nmol/L), and (D) and (H) formononetin + everolimus. p<0.05 versus formononetin or everolimus alone.

(a)

(b)