Migration and Proliferation Effects of Thymoquinone-Loaded Nanostructured Lipid Carrier (TQ-NLC) and Thymoquinone (TQ) on In Vitro Wound Healing Models

Table 3

Percentage of healthy, apoptotic, and necrotic cells of 3T3 treated with TQ as determined by flow cytometry.

		24 h	48 h	72 h
Control	Healthy cells	93.6 ± 0.4	91.4 ± 0.1	89.8 ± 0.6
	Early apoptosis	0.5 ± 0.1	1.6 ± 0.1	3.3 ± 0.8
	Late apoptosis	5.4 ± 0.4	5.5 ± 0.2	5.4 ± 0.1
	Necrosis	0.5 ± 0.1	1.5 ± 0.0	1.5 ± 0.1
3 μM	Healthy cells	94.6 ± 0.4	93.7 ± 0.1	90.3 ± 0.6
	Early apoptosis	0.5 ± 0.1	1.2 ± 0.1	2.6 ± 0.4
	Late apoptosis	3.7 ± 0.7	3.4 ± 0.2	6.2 ± 0.2
	Necrosis	1.2 ± 0.3	1.7 ± 0.0	0.9 ± 0.0
6 μM	Healthy cells	94.8 ± 0.7	91.4 ± 0.2	89.6 ± 0.7
	Early apoptosis	0.3 ± 0.1	1.0 ± 0.2	1.9 ± 0.2
	Late apoptosis	4.6 ± 0.4	6.6 ± 0.1	7.5 ± 0.9
	Necrosis	0.3 ± 0.2	1.0 ± 0.1	1.0 ± 0.4
12 μM	Healthy cells	91.3 ± 0.4	90.2 ± 0.2	87.9 ± 2.1
	Early apoptosis	0.6 ± 0.0	1.2 ± 0.2	2.1 ± 0.3
	Late apoptosis	6.9 ± 0.2	7.3 ± 0.4	8.9 ± 1.9
	Necrosis	1.2 ± 0.2	1.3 ± 0.4	1.1 ± 0.1