Chaihu-Shugan-San Reinforces CYP3A4 Expression via Pregnane X Receptor in Depressive Treatment of Liver-Qi Stagnation Syndrome

Figure 1

Timeline of the experiment. Mice were randomly divided into eight groups: In Liver-Qi stagnation: control, model, 4 g/kg of CSS and 12 g/kg of CSS; in liver stagnation and spleen deficiency: control, model, 4 g/kg CSS and 12 g/kg CSS. CSS or distilled water was given once per day 14 days after induction of depression. Weight, FST, and TST were assessed on days 0, 21, and 28. The mice were sacrificed, and liver microsomes were used to evaluate CYP450 expression after 28 days by Western blot analysis and RT-qPCR.