Salvianolic Acid B Attenuates Apoptosis of HUVEC Cells Treated with High Glucose or High Fat via Sirt1 Activation
Figure 2
SAB reverses the inhibitory effects of high glucose or high fat (PA) on HUVEC cells proliferation and apoptosis. (a) Effect of glucose (10, 20, and 30 mM) and PA (0.25, 0.5, and 0.75 mM) on the percentage of survival in HUVECs at 4, 12, and 24 hours by MTT assay; (b) effect of HG (30 mM) or PA (0.75 mM) with SAB (100, 200, and 400 mg/L) on cells viabilities; p <0.001 versus control group, #p <0.05 versus HG or PA group, ## p <0.01 versus HG or PA group, and ### p <0.001 versus HG or PA group. (c) Effect of SAB on apoptosis induced by HG or PA in HUVECs after Annexin V and PI double staining; p <0.001; ### p <0.001. (d) JC-1 staining assay for mitochondria membrane potential. SAB remained mitochondria membrane potential in HG- or PA-induced HUVEC cells; p <0.001; #p <0.05, ## p <0.01.