Optimization Extraction of Shikonin Using Ultrasound-Assisted Response Surface Methodology and Antibacterial Studies

Table 3

ANOVA of response surface quadratic model analysis for the extraction yield.

Source	Sum of squares	Degrees of freedom	Mean square	F-value	p value prob > F
Model	0.051	14	3.67E − 03	51.64	<0.0001^a
A	0.011	1	0.011	154.72	<0.0001
B	2.35E − 03	1	2.35E − 03	33.14	<0.0001
C	2.41E − 05	1	2.41E − 05	0.34	0.5695
D	4.32E − 04	1	4.32E − 04	6.09	0.0271
AB	3.42E − 04	1	3.42E − 04	4.82	0.0454
AC	3.61E − 04	1	3.61E − 04	5.09	0.0406
AD	8.10E − 05	1	8.10E − 05	1.14	0.3035
BC	3.60E − 05	1	3.60E − 05	0.51	0.488
BD	1.23E − 05	1	1.23E − 05	0.17	0.6841
CD	6.00E − 04	1	6.00E − 04	8.46	0.0115
A^2	0.029	1	0.029	404.78	<0.0001
B^2	0.011	1	0.011	160.64	<0.0001
C^2	9.81E − 04	1	9.81E − 04	13.83	0.0023
D^2	6.10E − 03	1	6.10E − 03	86	<0.0001
Residual	9.94E − 04	14	7.10E − 05
Lack of fit	8.76E − 04	10	8.76E − 05	2.99	0.1512^b

^aSignificant. ^bNot significant.