Feng-Liao-Chang-Wei-Kang Combined with 5-Fluorouracil Synergistically Suppresses Colitis-Associated Colorectal Cancer via the IL-6/STAT3 Signalling Pathway

Figure 3

FLCWK-enhanced 5-FU inhibited the IL-6/STAT3 pathway in vitro. HCT116 cells were pretreated with different concentrations of FLCWK and/or 5-FU for 1 h followed by stimulation with 40 ng/mL IL-6 for 24 h. (a) After treatment, total protein was extracted, STAT3 and phosphorylated STAT3, Bcl-2, CyclinD1, CDK4, and β-actin were detected by western blot. (b) STAT3 small interfering RNA was transfected into HCT116 cells. The cells were cultured in medium for 72 h after treatment cells were pretreated with various concentrations of FLCWK and/or 5-FU for 1 h followed by stimulation with 40 ng/mL IL-6 for 24 h. Phosphorylated STAT3 was detected by western blot. (c) Total protein of IL-6 and IL-1β was detected by western blot (d, e). The messenger RNA levels of STAT3, Bcl-2, CyclinD1, IL-6, and IL-1β were measured by Q-PCR. Values are mean ± SD of 3 separate experiments performed. ; vs blank group; ; vs IL-6 group; ; vs 5-FU group.

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