N-Butanol Extract of Gastrodia elata Suppresses Inflammatory Responses in Lipopolysaccharide-Stimulated Macrophages and Complete Freund’s Adjuvant- (CFA-) Induced Arthritis Rats via Inhibition of MAPK Signaling Pathway

Figure 6

Effects of different doses of BGE on the MAPK signaling pathway. (a) Representative expression of p-38, p-p38, ERK, and p-ERK obtained from ankle joints of several groups was detected by western blot analysis. (b) Quantitative analysis of gray value of p-p38 and p-ERK was performed in several groups with β-actin as loading control. Data represent the mean ± SD of three independent experiments. (c) Immunoblot analysis of expression of p-38, p-p38, ERK and p-ERK in RAW264.7 mouse macrophage treated with BGE (10, 20, and 40 μg/mL)and LPS (1 μg/mL) for 24 h. (d) Quantitative analysis of gray value of p-p38 and p-ERK was performed in several groups with β-actin as loading control. Data represent the mean ± SD of three independent experiments. , compared with the normal group. , , compared with the CFA/LPS group.

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