Photoaging Protective Effects of Ranunculus bulumei Methanol Extract

Figure 3

Effects of Rb-ME on photoaging responses in UVB-irradiated HaCaT cells. (a) HaCaT cells were irradiated with UVB (30 mJ/cm²) and then treated with or without Rb-ME (50 or 100 μg/mL) for 24 h The mRNA levels of MMP-9, COX-2, SIRT1, and type-1 procollagen were analyzed by RT-PCR. (b) The promoter-binding activity of the transcription factor Col1A1 was determined using a luciferase reporter gene assay. HEK293 cells were transfected with plasmids driving the expression of Col1A1-Luc (1 μg/mL), β-gal (as a transfection control), and FLAG-Smad3 (1 μg/mL) and then treated with or without Rb-ME (50 or 100 μg/mL) for 24 h. Luciferase activity was measured using a luminometer. (c) HaCaT cells were treated with Rb-ME (25 to 200 μg/mL) or retinol (10 μg/mL) for 24 h. The mRNA levels of FLG, TGM, HAS-1, HAS-2, and HAS-3 were analyzed by RT-PCR. compared to the normal group or compared to the control group.

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