Photoaging Protective Effects of Ranunculus bulumei Methanol Extract

Figure 5

Antimelanogenesis effect of Rb-ME in α-MSH-treated B16F10 cells. (a, b) The levels of melanin secretion and contents in B16F10 cells treated with α-MSH (100 nM) in the presence or absence of Rb-ME (50 to 400 μg/mL) or arbutin (1 mM) for 48 h were then determined. (c, d) The effect of Rb-ME (200 to 800 μg/mL) or kojic acid (100 or 300 μM) on mushroom tyrosinase activity was analyzed by measuring the activity of purified tyrosinase. (e) B16F10 cells treated with α-MSH (100 nM) in the presence or absence of Rb-ME (200 or 400 μg/mL) or arbutin (1 mM) for 24 h. The mRNA levels of tyrosinase, MITF, TYRP-1, and TYRP-2 were then determined by RT-PCR. compared to the normal group. compared to the control group and compared to the control group.

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