Amaranthus mangostanus Inhibits the Differentiation of Osteoclasts and Prevents Ovariectomy-Induced Bone Loss

Figure 2

Amaranthus mangostanus (AM) inhibited receptor activator of nuclear factor-κB (NF-κB) ligand- (RANKL-) induced osteoclastogenesis without cytotoxicity in vitro. (a) Effects of AM on cell viability. RAW 264.7 cells were cotreated with RANKL (50 ng/mL) and AM (0–100 μg/mL) for 4 days. (b) RAW 264.7 cells were treated with indicated concentrations of AM followed by RANKL (50 ng/mL) stimulation for 4 days. (c) Tartrate-resistant acid phosphatase- (TRAP-) positive cells with more than three nuclei were considered osteoclast; n = 3; compared with control group.

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