Amaranthus mangostanus Inhibits the Differentiation of Osteoclasts and Prevents Ovariectomy-Induced Bone Loss

Figure 3

Amaranthus mangostanus (AM) suppressed receptor activator of nuclear factor-κB (NF-κB) ligand- (RANKL-) induced expression of osteoclast-specific genes. (a)–(d) RAW 264.7 cells were cultured with RANKL (50 ng/mL) at various concentrations for 4 days, and osteoclast-specific genes (cathepsin K, TRAP, Dc-stamp, and nuclear factor of activated T-cells (NFATc1)) were analyzed using real-time polymerase chain reaction (PCR). RNA expression levels were normalized to expression levels of glyceraldehyde 3-phosphate dehydrogenase (GAPDH). (e) RAW 264.7 cells were cultured with RANKL (50 ng/mL) with various concentrations for 4 days and osteoclast-specific gene NFATc1 was analyzed using Western blotting; n = 3; compared with control group, and compared with RANKL treatment group.

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