A Natural Compound Mixture Containing Arctigenin, Hederagenin, and Baicalein Alleviates Atopic Dermatitis in Mice by Regulating HPA Axis and Immune Activity

Figure 6

Effects of ADM on proinflammatory factor production and MAPK activation in TI-stimulated HaCaT cells. HaCaT cells were pretreated with ADM (10, 30 μM) for 1 h and then stimulated with TI (TNF-α plus IFN-γ, 10 ng/ml each) for 24 h (a) TARC/CCL17, MDC/CCL22, IL-6, and ICAM-1 levels in culture supernatants were determined using commercial ELISA kits. (b) Effects of ADM on cell viability were assessed using an XTT assay. HaCaT cells were treated with ADM for 24 h at the indicated concentrations. Effects of ADM MAPK activation in TI-stimulated HaCaT cells HaCaT cells were pretreated with ADM (10, 30 μM) for 1 h and then stimulated with TI for 30 min. (c) Protein expressions of p-ERK, p-p38, and p-JNK were assessed by western blot, and band intensities were normalized versus β-actin. (d) HaCaT cells were pretreated with U0126, SB202190, and SP600125 (10 μM) for 1 h and then stimulated with TI for 24 h The TARC/CCL17 and MDC/CCL22 levels in the culture supernatants were determined using commercial ELISA kits. Results are presented as mean ± SD (n = 3 per experiment). vs. normal controls; vs. TI-treated cells.

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