System Prediction and Validation of TCM for Chronic Myeloid Leukemia Treatment from the Perspective of Low-Toxicity Chemotherapy: A Stilbene α-Viniferin Has a Proapoptotic Effect on K562 Cells via the Mitochondrial Pathway

Figure 5

The images in (a) show the morphological changes of K562 cells treated with 0–128 μg·mL⁻¹α-viniferin for 24 h (magnified by 200x); the images in (b–d) show the morphological changes in the k562 cell nucleus with Hoechst staining; (b, c) K562 cells treated with 0∼32 μg·mL⁻¹α-viniferin for 24 h (magnified by 200x); (d) K562 cells treated with H₂O₂ for 24 h (magnified by 200x); the images in (e–g) show differences in k562 cell apoptosis and necrosis with AO/EB staining; (e, f) K562 cells treated with 0∼32 μg·mL⁻¹α-viniferin for 24 h (magnified by 200x); (g) K562 cells treated with H₂O₂ for 24 h (magnified by 200x); the images show ultrastructural features of a representative control cell (h) and the morphological features of apoptosis in K562 cells treated with 32 μg·mL⁻¹ α-viniferin (i, j) by electron microscopy; (h) K562 cells without α-viniferin treatment (magnified by 5,000x). K562 cells treated with 32 μg·mL⁻¹ for 24 h; (i) magnified by 6,000x; (j) magnified by 12,000x).

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