Herb-Drug Interaction: Application of a UPLC-MS/MS Method to Determine the Effect of Polygonum capitatum Extract on the Tissue Distribution and Excretion of Levofloxacin in Rats

Table 3

Precision and accuracy of LVFX in rat liver tissues, urine, and feces (n = 5).

Biosamples	Spiked concentration (ng/mL)	Intraday			Interday
		Calculated concentration (ng/mL)	Precision (RSD, %)	Accuracy (%)	Calculated concentration (ng/mL)	Precision (RSD, %)	Accuracy (%)
LVFX in liver tissue	10.02	9.33 ± 1.03	11.03	93.07	9.18 ± 0.55	5.94	91.59
	100.20	104.61 ± 3.89	3.72	104.40	103.86 ± 4.09	3.94	103.65
	5010.00	4815.20 ± 158.68	3.30	96.11	4803.63 ± 111.96	2.33	95.88
LVFX in urine	10.02	9.49 ± 0.85	8.99	94.67	10.46 ± 0.51	4.85	104.35
	200.40	209.45 ± 10.49	5.01	104.52	207.55 ± 12.98	6.25	103.57
	4008.00	4183.48 ± 102.27	2.44	104.39	3875.60 ± 97.53	2.52	96.70
LVFX in feces	10.02	9.54 ± 0.86	9.03	95.17	10.54 ± 0.73	6.90	105.16
	20.04	20.56 ± 1.16	5.66	102.58	19.54 ± 0.91	4.64	97.53
	200.40	215.78 ± 13.68	6.34	107.67	190.05 ± 20.49	10.78	94.83