Combined Extracts of Epimedii Folium and Ligustri Lucidi Fructus with Budesonide Attenuate Airway Remodeling in the Asthmatic Rats by Regulating Apoptosis and Autophagy

Figure 5

Effects of bud and EEL on Caspase-3 and cleaved Caspase-3. The protein area (a) and IOD (b) of Caspase-3 were measured by immunofluorescence staining. (c) The mRNA expressions of Caspase-3 were measured by qPCR analysis with β-actin as an internal control. (d) Representative WB photographs of Caspase-3 and cleaved Caspase-3 were viewed, and β-actin was used for normalization. (e) Summarized data of mean density of cleaved Caspase-3 and Caspase-3 protein expression in lung tissues measured by WB, normalized to β-actin. (f) Representative immunofluorescence images of Caspase-3 (×200). Data are represented as mean ± SD; n = 6. compared with control group; and compared with asthma group; compared with Bud group; compared with EEL group.

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