Anticancer Effect of Mountain Ginseng on Human Breast Cancer: Comparison with Farm-Cultivated Ginseng
Effect of MGE on expression of apoptosis-related proteins. MCF-7 cells were seeded on a 6-well plate (5 × 104 cells/well) and then further incubated. Next day, the cells were incubated with MGE for 24 h. The expression of Bcl-2, Bax, cytochrome c cleaved caspase-3, or β-actin and caspase-3/7 activity was determined as described in the Materials and Methods section. Similar results for immunoblot analysis were obtained in three independent experiments. Data of caspase-3/7 activity are expressed as the mean ± SD values of quadruple determinations. and versus the control group. (a) Bcl-2, Bax, cytochrome c, and cleaved caspase-3; (b) caspase-3/7 activity.
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