Curcumin Inhibits Hepatocellular Carcinoma via Regulating miR-21/TIMP3 Axis

<div>Effects of miR-21 inhibition and/or TIMP3 silencing on cell proliferation in HCC cell lines exposed to curcumin. (a, b) HepG2 and HCCLM3 cells were transfected with miR-21 inhibitor or si-TIMP3 sequence for 24 h. RT-qPCR was applied to quantify the expression of miR-21 and TIMP3. <svg height="15.672pt" id="M12" style="vertical-align:-3.834pt" version="1.1" viewbox="-0.0498162 -11.838 51.9505 15.672" width="51.9505pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M724 428H489V396C558 388 570 377 552 332C533 288 517 255 494 220C431 283 362 343 329 370C366 395 388 414 407 430C430 449 449 478 449 518C449 598 382 657 300 657C209 657 133 590 133 499C133 444 161 403 204 363C116 299 54 261 54 169C54 81 127 -14 255 -14C327 -14 396 20 468 116C500 84 539 36 553 22C565 8 621 -14 677 -19L684 -8C652 49 565 147 521 191C557 244 580 284 607 323C640 373 654 388 724 396V428ZM288 619C337 619 373 573 373 516C373 465 347 427 300 392C236 437 207 471 207 522C207 579 241 619 288 619ZM282 57C214 57 142 105 142 197C142 246 168 291 233 338C329 262 390 203 441 146C409 101 349 57 282 57Z"></path></g><g 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Cell viability was evaluated by CCK-8 assay. 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557C201 555 205 550 205 499V114C205 42 199 34 109 27V0H384Z"></path></g></svg> vs. control group; <svg height="15.672pt" id="M15" style="vertical-align:-3.834pt" version="1.1" viewbox="-0.0498162 -11.838 52.5169 15.672" width="52.5169pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M611 626L575 651L170 -9L204 -35L611 626ZM200 635C93 635 50 532 50 449C50 365 94 264 199 264C306 264 349 365 349 450C349 534 304 635 200 635ZM199 601C264 601 273 501 273 450S264 298 200 298C139 298 126 398 126 450S139 601 199 601ZM590 359C485 359 440 256 440 173C440 89 485 -12 589 -12C694 -12 738 89 738 173C738 256 694 359 590 359ZM589 325C653 325 662 225 662 173C662 124 653 22 590 22C529 22 516 122 516 174C516 227 529 325 589 325Z"></path></g><g transform="matrix(.013,0,0,-0.013,7.678,.966)"><path d="M600 480C600 590 528 650 384 650H143L137 622C222 614 225 607 210 531L130 127C113 41 106 36 23 28L17 0H294L300 28C204 36 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557C201 555 205 550 205 499V114C205 42 199 34 109 27V0H384Z"></path></g></svg> vs. curcumin group; and <svg height="15.3614pt" id="M17" style="vertical-align:-3.833899pt" version="1.1" viewbox="-0.0498162 -11.5275 49.7858 15.3614" width="49.7858pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M457 455H370L403 612H360L326 455H218L252 612H210L175 455H77L68 394H161L132 257H31L20 196H117L77 19H120L159 196H268L230 19H272L311 196H402L412 257H325L355 394H447L457 455ZM313 394L283 257H175L204 394H313Z"></path></g><g transform="matrix(.013,0,0,-0.013,4.946,.966)"><path d="M600 480C600 590 528 650 384 650H143L137 622C222 614 225 607 210 531L130 127C113 41 106 36 23 28L17 0H294L300 28C204 36 195 42 212 127L243 284L314 263C327 263 339 263 352 264C465 271 600 337 600 480ZM508 481C508 351 402 304 329 304C289 304 265 311 250 317L295 559C302 594 310 606 323 611C335 616 350 619 367 619C455 619 508 573 508 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Evidence-Based Complementary and Alternative Medicine

fig6

Figure 6

Figure 6: Curcumin Inhibits Hepatocellular Carcinoma via Regulating miR-21/TIMP3 Axis 