Phenylethanol Glycosides Protect Myocardial Hypertrophy Induced by Abdominal Aortic Constriction via ECE-1 Demethylation Inhibition and PI3K/PKB/eNOS Pathway Enhancement

Table 2

The primer sequences used for real-time PCR assay.

Genes	Forward primer (5′-3′)	Reverse primer (3′–5′)
ANP	GAGAGCGGACTAGGCTGCAA	TCAGTGGCAAlGCGACCAA
BNP	CGGATTGGCGCAGTCAGTCG	AGAGCCGCAGGCAGAGTCAG
β-MHC	GTGCCAATGACGACCTGAAGGAG	CTGGTTGATGAGGCTGGTGTTCTG
ECE-1	TCATCGGCTCACTCTCCAACTCC	CCTTCTTCCACCTGTGTTCTCTGC
β-Actin	TGTCACCAACTGGGAGGATA	GGGGTGTTGAAGGTCTCAAA

RNA expression levels were measured by SYBR on 7500 Real-Time PCR System (Applied Biosystems, USA). Each experiment was conducted in triplicate.