In Vitro and Randomized, Double‐Blind, Placebo‐Controlled Trial to Determine the Efficacy and Safety of Nine Antiacne Medicinal Plants

Table 2

The effect of Linum pubescens (LP), Nigella sativa (NS), Hypericum triquetrifolium (HT), Ferula hermonis (FH), Cappris spinose (CS), Inula helenium (IH), Eruca sativa (ES), Saponaria officinalis (SO), Urtica dioica (UD), and Solanum nigrum (SN) extracts on sebum production in sebaceous glands organ culture was measured though assessment of acetate incorporation into sebaceous gland total lipids in the absence and presence of ethanolic extracts. Each result represents an average of nine repeated experiments.

Extracts (mg/ml)	LP	NS	HT	FH	CS	IH	ES	SO	UD
0.00	1.00	1.00	1.00	1.00	1.00	1.00	1.00	1.00	1.00
0.05	0.59	0.91
0.06			1.00	0.71	0.89	0.50	0.81	0.18	0.67
0.11	0.87
0.19			0.83	0.51	0.89	0.34	0.51	0.33	0.75
0.33	0.75
0.5		0.80
0.56			0.29	0.56	0.57	0.08	0.60	0.00	0.41
1.00	0.35
1.67			0.18	0.23	0.47	0.00	0.08	0.00	0.66
3.00	0.13
5.00		0.03	0.03	0.02	0.22	0.01	0.03	0.04	0.26
r	−0.83	−1.00	−0.96	−0.94	−0.94	−0.94	−0.94	−0.80	−0.89
	0.05	0.05	0.01	0.01	0.01	0.01	0.01	0.10	0.05
#/point	5	10	5	5	2	2	2	2	2
EC50 (mg/ml)	0.67	1.28	0.43	0.57	1.11	0.09	0.49	0.03	1.54
STDEV	0.41	0.30	0.09	0.33	0.19	0.02	0.22	0.01	1.18

r: Spearman matched pair rank correlation coefficient; : probability that r is random (one tail); #/point: number of glands used per data point; EC50: calculated concentration (mg/ml) at which 50% of the untreated control lipid incorporation is obtained; EC50: 95% confidence limits for EC50 (mg/ml) (^∗P < 0.05 significant as compared to control).