UHPLC-ESI-Orbitrap-MS Analysis of Biologically Active Extracts from Gynura procumbens (Lour.) Merr. and Cleome gynandra L. Leaves

Table 1

The total phenolic content (TPC), DPPH scavenging activity, nitric oxide (NO) inhibition activity, and cell cytotoxicity (MTT) of the G. procumbens and G. gynandra extracts.

Extracts % EtOH	G. procumbens				C. gynandra
	TPC (mg GAE/100 mgde)	DPPH (IC50) μg/mL	NO inhibition (IC50) μg/mL	MTT (%) at 1000 μg/mL	TPC (mg GAE/100 mgde)	DPPH (IC50) μg/mL	NO inhibition (IC50) μg/mL	MTT (%) at 1000 μg/mL
0	1.44 ± 0.03^a	124.37 ± 6.28^a	117.19 ± 10.50^a	96.31 ± 3.06	3.38 ± 0.06^a	62.01 ± 5.84^a	210.06 ± 12.25^a	ND
20	1.58 ± 0.32^a	317.41 ± 8.32^b	96.77 ± 7.09^b	97.91 ± 3.84	3.46 ± 0.26^a	40.36 ± 5.09^b	94.83 ± 10.49^b	ND
50	3.81 ± 0.04^b	66.28 ± 3.95^c	82.03 ± 8.95^c	95.76 ± 8.01	1.51 ± 0.18^b	276.92 ± 6.42^c	97.11 ± 9.09^b	ND
70	3.15 ± 0.19^b	131.92 ± 8.99^a	22.91 ± 6.75^d	93.54 ± 8.73	2.74 ± 0.28^c	304.64 ± 9.02^d	66.01 ± 7.75^c	91.53 ± 3.25
100	5.91 ± 0.62^c	63.73 ± 2.90^c	25.20 ± 6.19^d	91.71 ± 7.72	3.71 ± 0.26^a	236.95 ± 7.96^c	60.75 ± 6.32^c	89.11 ± 4.77
Quercetin	—	1.73 ± 0.12	—	—	—	1.73 ± 0.12	—	—
Curcumin	—	—	3.59 ± 0.28	—	—	—	3.59 ± 0.28	—

Values are the mean ± standard deviation of six biological replicates. Bold numbers to show extracts with the best activity for related assays. GAE: gallic acid equivalent; de: dried extract; EtOH: ethanol; ND: not detected. Each different superscript letter within the same assay indicates a statistically significant different ().