Evidence-Based Complementary and Alternative Medicine

Research Article

Metabolomics of the Protective Effect of Ampelopsis grossedentata and Its Major Active Compound Dihydromyricetin on the Liver of High-Fat Diet Hamster

Table 1

Characterization of compounds detected in Ampelopsis grossedentata extract by HPLC-DAD-ESI-IT-TOF-MSⁿ.


No.	t_R (min)	Formula	PI pred. (Da)	PI meas. (Da)	Error (ppm)	Major fragment ions	Identification

1.	2.725	C₁₅H₁₂O₆	289.0707	289.0711^a	1.51	275.0607, 245.0654, 203.0556, 185.0416	Aromadendrol
2.	2.725	C₆H₁₂O₇	195.0510	195.0508^b	1.15	177.0417, 159.0328, 113.0227	Gluconic acid
3.	2.725	C₂₁H₁₆O₆	365.1020	365.1002^a	4.85	305.0793, 203.0556, 185.0416	—
4.	2.950	C₁₆H₁₄O₆	301.0718	301.0744^b	8.73	173.0267	Hesperetin
5.	4.230	C₁₃H₁₆O₁₀	333.0816	333.0814^a	0.67	169.0153, 125.0248	Galloy-β-D-glucose
6.	5.487	C₇H₆O₅	169.0412	169.0148^b	3.25	125.0288	Gallic acid
7.	6.490	C₂₁H₂₂O₁₃	481.0988	481.0990^b	0.49	463.0852, 319.0421, 301.0252, 193.0132	Ampelopsin-4′-O-β-D-glucopyranoside
8.	8.318	C₂₃H₃₂O₁₂	499.1821	499.1792^b	5.80	385.1851, 205.1231, 161.0500	Isopentenyl benzoic acid-4-O-xylosylglucoside
9.	10.770	C₈H₈O₅	183.0299	183.0295^b	2.16	183.0318	Methyl gallate
10.	16.545	C₂₂H₂₂O₁₀	491.1195	491.1180^c	3.36	283.0573, 268.0368, 239.0307	Calycosin-7-O-β-D-glucoside
11.	16.887	C₉H₁₀O₅	197.0455	197.0411^b	7.31	—	Ethyl gallate
12.	20.387	C₁₅H₁₂O₈	319.0459	319.0443^b	5.13	301.0335, 193.0140, 179.0056	Dihydromyricetin
13.	22.912	C₂₁H₂₀O₁₃	481.0977	481.0956^a	4.31	319.0408, 165.0210	Myricetin-3-O-β-D-galactopyranoside
14.	25.028	C₂₇H₃₆O₇	471.2388	471.2383^b	1.12	237.0389, 175.0377	—
15.	25.778	C₂₀H₁₈O₁₂	449.0725	449.0711^b	9.57	273.0467, 245.0405, 217.0501	Myricetin-3′-O-β-D-xyloside
16.	26.003	C₁₅H₁₀O₈	319.0448	319.0429^a	6.11	273.0398, 245.0447, 217.0471, 165.1365, 153.0194	Myricetin
17.	26.003	C₂₇H₃₀O₁₆	611.1607	611.1664^a	9.40	321.0481, 301.0311, 165.0195, 153.0194	Rutin
18.	26.062	C₂₁H₂₀O₁₂	463.0882	463.0868^b	3.02	463.0851, 317.0285, 287.0180, 271.0246	Myricitrin
19.	26.062	C₂₁H₂₀O₁₂	463.0882	463.0868^b	3.02	316.0261, 301.0307, 271.0246	Quercetin-3-O-β-D-glucoside
20.	26.287	C₂₂H₂₆O₈	417.1555	417.1536^b	4.52	318.0308, 273.0726, 262.0683	Grossedentatasin
21.	27.328	C₂₁H₂₀O₁₁	447.0933	447.0940^b	1.60	447.0878, 301.0307, 271.0264	Quercetin-3-rhamnoside
22.	27.553	C₁₅H₁₀O₅	269.0455	269.0433^b	8.32	241.0401, 225.0529	Emodin
23.	27.920	C₁₅H₁₂O₇	303.0510	303.0490^a	6.66	287.0542, 153.0206, 123.0509	Dihydroquercetin
24.	28.303	C₂₁H₂₀O₁₁	447.0933	447.0963^a	0.70	285.0380, 255.0276, 227.0326, 151.0052	Astragalin
25.	28.362	C₂₁H₂₀O₁₀	433.1129	433.1097^a	7.46	165.0204, 153.0155, 147.0379, 129.0772	Kaempeerol-3-O-glucorhamnoside
26.	28.362	C₁₅H₁₀O₆	287.0550	287.0518^a	11.24	241.0500, 213.0587, 165.0240, 153.0155, 121.0289	Kaempferol
27.	29.637	C₁₅H₁₀O₅	269.0455	269.0433^b	8.32	227.0373, 183.0453, 149.0235	Apigenin
28.	29.795	C₁₅H₁₀O₇	301.0354	301.0334^b	6.54	273.0354, 229.0490, 165.0176, 137.0275	Quercetin
29.	30.678	C₂₉H₅₀O	415.3934	415.3909^a	6.14	367.2291, 317.2050171.1095	β-Sitosterol
30.	31.563	C₂₈H₃₆O₁₃	579.2083	579.2124^b	7.04	271.1662, 245.1892, 135.0843	Grossedentataside
31.	32.908	C₂₉H₄₈O	411.3632	411.3628^b	1.07	393.2614, 368.3089, 213.1123	Stigmasterol

Note: ^a[M + H]^＋; ^b[M − H]⁻; ^c[M + COOH]⁻.