Transcriptome Analysis Reveals the Effects of Troxerutin and Cerebroprotein Hydrolysate Injection on Injured Spinal Cords in Rats

Figure 5

Assessment of sequencing library quality. As the number of repetitions increases, the number of log 10 sequences corresponding to the number of repetitions decreases, and the overall trend of each group is extended linearly and smoothly as shown in (a)–(d). The density of different distances between the double-ended reads on the reference genome ranges from -150 bp to 250 bp, and the main peak of each sample is near 0 bp in (e)–(h). The peak shape is wide, indicating that the length of the insert is more discrete. The gene expression saturation curve of different expression levels is illustrated with a different color for each line in (i)–(l): red lines represent 0–0.3, yellow lines represent 0.3–0.6, green lines represent 0.6–3.5, light blue lines represent 3.5–15, and purple lines represent ≥60. The percentage of effective alignment reads increases along with the proportion of the expression with an error rate of under 15% compared to the final value: the closer the proportion value is to 1, the more saturated the expression level tends to be. The sooner the plateau is reached, the higher is the expression level of the gene in the sample.

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