Dehydrocorydaline Accounts the Majority of Anti-Inflammatory Property of Corydalis Rhizoma in Cultured Macrophage

Figure 3

CR extracts inhibit protein expressions of proinflammatory cytokines. Cultured RAW 264.7 cells in 24-well plates were treated with different doses of CR extracts by water and different fractions of ethanol (25%, 50%, 75%, and 100%) for 2 hours, followed by added LPS (20 ng/mL) and cultured for further 6 hours. The doses of CR extracts from 5 to 20 mg/mL were chosen as noncytotoxicity according to their cell viability in Supplement Figure 1. The cultured medium was collected and tested for levels of proinflammatory cytokines by ELISA. Data are expressed as mean ± SEM, n = 3, each with triplicate samples. and , compared to model control (LPS but no CR extracts).

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